rabbit polyclonal anti bovine igm hrp  (Bethyl)

Journal: Toxics

Article Title: Effects of Diethylstilbestrol on the Structure and Function of the Spleen in Male Golden Hamsters.

doi: 10.3390/toxics13050397

Figure Lengend Snippet: Figure 6. Effects of DES on estrogen receptor expression in the spleen of adult male golden hamsters: (A–C) Immunohistochemical localization of ERα, ERβ, and GPER in the splenic tissue. Triangles indicate estrogen receptor-positive cells. Scale bar = 50 µm. (D–F) mRNA expression levels of Esr1, Esr2, and Gper in the spleen. (G–I) Protein expression levels of ERα, ERβ, and GPER in the spleen. Different letters (e.g., a, b, c) indicate statistically significant differences between groups (p < 0.05). Groups sharing at least one common letter are no different from each other (p > 0.05). Data are expressed as mean ± SEM.

Article Snippet: The sections were then incubated overnight at 4 ◦C with primary antibodies, including mouse anti-human Proliferating Cell Nuclear Antige (PCNA) monoclonal antibody (1:2000, ZSGBBIO, Beijng, China), rabbit anti-mouse caspase-3 polyclonal antibody (1:200, ZSGB-BIO, Beijng, China), rabbit anti-mouse inducible nitric oxide synthase (iNOS) polyclonal antibody (1:200, ZSGB-BIO, Beijng, China), mouse anti-estrogen receptor α (ERα) monoclonal antibody (1:100, Enzo Life Sciences, Inc., Farmingdale, NY, USA), rabbit anti-mouse estrogen receptor beta (ERβ) polyclonal antibody (1:300, Invitrogen, Waltham, MA, USA), and rabbit anti-mouse G protein-coupled estrogen receptor (GPER) polyclonal antibody (1:200, Novus Biologicals, Centennial, CO, USA).

Techniques: Expressing, Immunohistochemical staining

Journal: Frontiers in Immunology

Article Title: Novel immunochromatographic test for rapid detection of anti-factor H autoantibodies with an assessment of its clinical relevance

doi: 10.3389/fimmu.2024.1527016

Figure Lengend Snippet: Characterization of IgM FH-FHAA complexes in samples with conflicting ELISA and ICT results. (A) Results of the analisis by the ICT of plasma samples from cases #18, #34 and #38, from a healthy control (IgG/IgM FHAA negative plasma) and from a strong positive plasma for IgG FHAA that was negative for IgM FHAA (H271B). (B) Plasma from these five samples was passed through an affinity column coated with a goat polyclonal anti-human IgM (Fc fragment) that specifically captures human IgM antibodies and the eluate for each sample tested for the presence of FH by ELISA. The results of these experiments show that while no FH was retained from the healthy control plasma nor the strong positive plasma for IgG FHAA, significant amounts of FH were eluted from the column in the case of the case of the three samples that tested positive for IgM FH-FHAA complexes in the ICT.

Article Snippet: As compared to Cassette A, Cassette C uses a rabbit polyclonal antibody anti-human IgM specific for the μ chain from Jackson Immunoresearch Europe (Ely, UK), which is coated onto the detection line of the nitrocellulose membrane.

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Control, Affinity Column